Recombinant COVID-19 N protein(Met1-Ala419(335Gly/Ala)), His-tagged, Biotinylated

• Cat.No.: N-2394C
• Product Overview: Recombinant COVID-19 Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419(335Gly/Ala)) was expressed in Insect Cells with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

Specification

• Species: Sars-CoV-2
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ala419(335Gly/Ala)
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, 500mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) consists of 430 amino acids and predicts a molecular mass of 47.08 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin