Recombinant COVID-19 N protein(R203K, G204R), His-tagged

• Cat.No.: N-709V
• Product Overview: Recombinant COVID-19 N protein(R203K, G204R)(QHO62115.1)(Met 1 - Ala 419), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Ala 419
• Tag: C-His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-1 ng/mL.
• Molecular Mass: The protein has a calculated MW of 47.4 kDa.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.