Recombinant COVID-19 NSP1 protein, His-tagged
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|Product Overview :||Recombinant COVID-19 NSP1 protein (Met1-Gly180) was fused to his-tag at the C-terminus and expressed in E.coli.|
|Description :||The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.|
|Predicted N Terminal :||Met|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Met1-Gly180|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|SDS-PAGE of NSP1-002V :||
COVID-19 NSP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Gene Name :||orf1ab|
|Official Symbol :||orf1ab|
|Gene ID :||1489680|
|Protein Refseq :||NP_828860.2|
|UniProt ID :||P0C6X7|