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Recombinant COVID-19 NSP1 protein, His-tagged

Cat.No. : NSP1-002V
Product Overview : Recombinant COVID-19 NSP1 protein (Met1-Gly180) was fused to his-tag at the C-terminus and expressed in E.coli.
  • Specification
  • Gene Information
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Description : The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
Source : E.coli
Species : COVID-19
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Gly180
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
Shipping : Powder,RT
Gene Name : orf1ab
Official Symbol : orf1ab
Synonyms : pp1ab
Gene ID : 1489680
Protein Refseq : NP_828860.2
UniProt ID : P0C6X7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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