Recombinant COVID-19 NSP7 protein, His-tagged (MALS verified) NSP7-198V

Recombinant COVID-19 NSP7 protein, His-tagged (MALS verified)

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Recombinant COVID-19 NSP7 protein, His-tagged (MALS verified)

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Cat.No. : NSP7-198V
Product Overview : Recombinant COVID-19 NSP7 protein (Ser1-Gln83) was fused to his-tag at the C-terminus and expressed in E.coli.
Description : During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
Source : E.coli
Species : COVID-19
Tag : His
Predicted N Terminal : Met
Form : Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 with glycerol as protectant.
Molecular Mass : The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Ser1-Gln83
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Shipping : Powder,RT
SDS-PAGE of NSP7-198V :

COVID-19 NSP7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS of NSP7-198V :

The purity of COVID-19 NSP7, His Tag was more than 90% and around 20-25 kDa verified by SEC-MALS.

Gene Name : orf1ab
Official Symbol : orf1ab
Synonyms : pp1ab
Gene ID : 1489680
Protein Refseq : NP_828865.1
UniProt ID : P0C6X7

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