Recombinant COVID-19 NSP7 protein, His-tagged (MALS verified)

• Cat.No.: NSP7-198V
• Product Overview: Recombinant COVID-19 NSP7 protein (Ser1-Gln83) was fused to his-tag at the C-terminus and expressed in E.coli.

Specification

• Description: During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
• Source: E.coli
• Species: COVID-19
• Tag: His
• Predicted N Terminal: Met
• Form: Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 with glycerol as protectant.
• Molecular Mass: The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Ser1-Gln83
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Shipping: Powder,RT

Gene Information

• Gene Name: orf1ab
• Official Symbol: orf1ab
• Synonyms: pp1ab
• Gene ID: 1489680
• Protein Refseq: NP_828865.1
• UniProt ID: P0C6X7