Recombinant COVID-19 NSP7 protein, His-tagged (MALS verified)
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
|Product Overview :||Recombinant COVID-19 NSP7 protein (Ser1-Gln83) was fused to his-tag at the C-terminus and expressed in E.coli.|
|Description :||During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.|
|Predicted N Terminal :||Met|
|Form :||Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 with glycerol as protectant.|
|Molecular Mass :||The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Ser1-Gln83|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|SDS-PAGE of NSP7-198V :||
COVID-19 NSP7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|SEC-MALS of NSP7-198V :||
The purity of COVID-19 NSP7, His Tag was more than 90% and around 20-25 kDa verified by SEC-MALS.
|Gene Name :||orf1ab|
|Official Symbol :||orf1ab|
|Gene ID :||1489680|
|Protein Refseq :||NP_828865.1|
|UniProt ID :||P0C6X7|