| Cat.No. : |
Spike-214V
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant COVID-19 S protein RBD ((N354D, D364Y), Arg319-Phe541) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293). |
| Description : |
It's been reported that COVID-19 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Source : |
HEK293 |
| Species : |
COVID-19 |
| Tag : |
His |
| Predicted N Terminal : |
Arg319 |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
Arg319-Phe541 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of Spike-214V : |
COVID-19 S protein RBD (N354D, D364Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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| Bioactivity-ELISA of Spike-214V : |
Immobilized Human ACE2, Fc Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S protein RBD (N354D, D364Y), His Tag with a linear range of 2-63 ng/mL (QC tested).
Immobilized COVID-19 S protein RBD (N354D, D364Y), His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-COVID-19-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (Routinely tested).
Immobilized COVID-19 S protein RBD (N354D, D364Y), His Tag at 2 μg/mL (100 μL/well) can bind Anti-COVID-19 Neutralizing Antibody, Human IgG1 with a linear range of 0.195-6.25 ng/mL (Routinely tested).
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| Bioactivity-SPR of Spike-214V : |
Human ACE2, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind COVID-19 S protein RBD (N354D, D364Y), His Tag with an affinity constant of 7.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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| Bioactivity-BLI of Spike-214V : |
Loaded Human ACE2, Fc Tag on Protein A Biosensor, can bind COVID-19 S protein RBD (N354D, D364Y), His Tag with an affinity constant of 6.32 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.