Active Recombinant SARS-CoV-2 S protein, His-tagged

• Cat.No.: Spike-190V
• Product Overview: Recombinant SARS-CoV-2 S protein(Arg319-Phe541), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: SARS-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: 319-541 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Human ACE-2 with a linear range of 0.001-2.96 ng/mL. 2. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 35.3 nM as determined in a SPR assay (Nicoya OpenSPR). 3. Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Human ACE-2 with a linear range of 0.001-1. 69 ng/mL.
• Molecular Mass: 25.94 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 95 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
• Publications:
  • Interfacial Water in the SARS Spike Protein: Investigating the Interaction with Human ACE2 Receptor and In Vitro Uptake in A549 Cells (2022)
  • Biomaterials and oxygen join forces to shape the immune response and boost SARS-CoV-2 vaccines (2020)
  • Prevalence of SARS-CoV-2 Antibodies in Children and Adults with Type 1 Diabetes (2021)
  • COVID-19 Vaccine Candidates Based on Modified Vaccinia Virus Ankara Expressing the SARS-CoV-2 Spike Protein Induce Robust T- and B-Cell Immune Responses and Full Efficacy in Mice (2021)
  • SARS-CoV-2 Spike Protein Destabilizes Microvascular Homeostasis (2021)
  • Biomaterials and Oxygen Join Forces to Shape the Immune Response and Boost COVID-19 Vaccines (2021)
  • Broadly neutralizing aptamers to SARS-CoV-2: A diverse panel of modified DNA antiviral agents (2023)

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1