Active Recombinant COVID-19 Spike protein receptor binding domain (RBD), His-tagged
Cat.No. : | Spike-190V |
Product Overview : | Recombinant COVID-19 Spike protein receptor binding domain (RBD) was fused to His-tag at C-terminus and expressed in human cells. |
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- Gene Information
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Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (RBD, His Tag) is 20-60 ng/mL. |
Molecular Mass : | The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.54 kDa. |
Protein Length : | Arg319-Phe541 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Publication : |
SARS-CoV-2 Spike Protein Destabilizes Microvascular Homeostasis (2021)
Biomaterials and Oxygen Join Forces to Shape the Immune Response and Boost COVID-19 Vaccines (2021)
Interfacial Water in the SARS Spike Protein: Investigating the Interaction with Human ACE2 Receptor and In Vitro Uptake in A549 Cells (2022)
Broadly neutralizing aptamers to SARS-CoV-2: A diverse panel of modified DNA antiviral agents (2023)
Biomaterials and oxygen join forces to shape the immune response and boost SARS-CoV-2 vaccines (2020)
Prevalence of SARS-CoV-2 Antibodies in Children and Adults with Type 1 Diabetes (2021)
COVID-19 Vaccine Candidates Based on Modified Vaccinia Virus Ankara Expressing the SARS-CoV-2 Spike Protein Induce Robust T- and B-Cell Immune Responses and Full Efficacy in Mice (2021)
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Products Types
◆ Lysates | ||
Spike-1058HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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