Recombinant COVID-19 Spike protein S1, Fc-tagged Spike-239V

Recombinant COVID-19 Spike protein S1, Fc-tagged

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Recombinant COVID-19 Spike protein S1, Fc-tagged

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Cat.No. : Spike-239V
Product Overview : Recombinant COVID-19 NSP8 protein (Val16-Arg685) was fused to Mouse IgG2a Fc tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source : HEK293
Species : COVID-19
Tag : Mouse IgG2a Fc
Predicted N Terminal : Val16
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 101.9 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Val16-Arg685
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of Spike-239V :

COVID-19 S1 protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of Spike-239V :

Immobilized Human ACE2, Fc Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S1 protein, Mouse IgG2a Fc Tag with a linear range of 0.4-6 ng/mL (QC tested).

Bioactivity-BLI of Spike-239V :

Loaded COVID-19 S1 protein, Mouse IgG2a Fc Tag on AMC Biosensor, can bind Human ACE2, His Tag with an affinity constant of 3.95 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Gene Name : S
Official Symbol : S
Synonyms : spike glycoprotein
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

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