Recombinant COVID-19 Spike RBD (A475V) protein, His-tagged

Cat.No. : Spike-388V
Product Overview : Recombinant COVID-19 Spike RBD (A475V) protein(YP_009724390.1)(Arg 319 - Lys 537), fused to His tag at the C-terminus, was expressed in HEK293.
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Description : It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source : HEK293
Species : COVID-19
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass : The protein has a calculated MW of 26.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Arg 319 - Lys 537
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE:
Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : S
Synonyms : Spike; S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD; COVID-19
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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