| Species : |
Sars-CoV-2 |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
Arg319-Phe541 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His is 5.0-25.0 ng/mL. |
| Molecular Mass : |
The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(Arg319-Phe541(E484Q))-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 35.1 kDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
