Species : |
Sars-CoV-2 |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Arg319-Phe541 |
Conjugation/Label : |
Biotin |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated, the EC50 of SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated is 20-160 ng/mL. |
Molecular Mass : |
The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
Purity : |
> 85 % as determined by SDS-PAGE. |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |