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Recombinant COVID-19 Spike S1 protein, His-tagged
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| Cat.No. : | Spike-237V |
| Product Overview : | Recombinant COVID-19 Spike S1 protein (Val16-Arg685) was fused to his-tag at the C-terminus and expressed in human 293 cells (HEK293). |
| Description : | It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Source : | HEK293 |
| Species : | COVID-19 |
| Tag : | His |
| Predicted N Terminal : | Val16 |
| Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : | The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : | Val16-Arg685 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >90% as determined by SDS-PAGE. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Shipping : | Powder,RT |
| SDS-PAGE of Spike-237V : |
COVID-19 S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
| Bioactivity-ELISA of Spike-237V : |
Immobilized COVID-19 S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.2-6 ng/mL (QC tested). 
Immobilized COVID-19 S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-COVID-19-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested). 
Immobilized COVID-19 S1 protein, His Tag at 1 μg/mL (100 μL/well) can bind Anti-COVID-19 Neutralizing Antibody, Human IgG1 with a linear range of 0.2441-7.8125 ng/mL (Routinely tested). |
| Bioactivity-BLI of Spike-237V : |
Loaded Human ACE2, Fc Tag on Protein A Biosensor, can bind COVID-19 S1 protein, His Tag with an affinity constant of 21.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Gene Name : | S |
| Official Symbol : | S |
| Synonyms : | spike glycoprotein |
| Gene ID : | 43740568 |
| Protein Refseq : | YP_009724390.1 |
| UniProt ID : | P0DTC2 |
◆ Recombinant Protein
| Spike-250V | Recombinant COVID-19 Spike protein, His/mFc-tagged | +Inquiry |
| Spike-192V | Recombinant COVID-19 (2019 novel coronavirus) Spike protein S1 subunit, Human IgG1 Fc-tagged | +Inquiry |
| Spike-19V | Recombinant COVID-19 (2019 novel coronavirus) Spike protein S1 subunit receptor binding domain (RBD), Mouse IgG1 Fc-tagged | +Inquiry |
| Spike-208V | Recombinant COVID-19 Full Length Spike Protein(Val16-Pro1213, R683A, R685A), His-tagged, active trimer (MALS verified) | +Inquiry |
| Spike-205V | Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S2, S2') protein | +Inquiry |
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◆ Lysates
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| Spike-001HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
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