Recombinant Cynomolgus ACVRL1 protein(Met1-Gln118), hFc-tagged

• Cat.No.: ACVRL1-2353C
• Product Overview: Recombinant Cynomolgus ACVRL1 (Met1-Gln118) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Gln118
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1-5 ng/ml.
• Molecular Mass: The recombinant cynomolgus ACVRL1 is a disulfide-linked homodimer. The reduced monomer comprises 338 amino acids and has a calculated molecular mass of 37.8 KDa.The apparent molecular mass of the protein is approximately 53 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.