Recombinant Cynomolgus C5 protein, His-tagged

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Recombinant Cynomolgus C5 protein, His-tagged

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Cat.No. : C5-681C
Product Overview : Recombinant Cynomolgus C5 (Gln19-Cys1676) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Source : HEK293
Species : Cynomolgus
Tag : His
Predicted N Terminal : Gln19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 70 kDa (β chain) and 116 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln19-Cys1676
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of C5-681C :

Cynomolgus Complement C5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of C5-681C :

Immobilized Cynomolgus Complement C5, His Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

Gene Name : C5
Official Symbol : C5
Synonyms : C5; complement component 5; complement C5; CPAMD4; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; FLJ17816; FLJ17822; MGC142298
Gene ID : 102125658
mRNA Refseq : XM_005580915.2
Protein Refseq : XP_005580972.1
UniProt ID : A0A2K5U1T1

Products Types

◆ Recombinant Protein

C5-0077HRecombinant Human C5 Protein, GST-Tagged+Inquiry
C5-575HRecombinant Human C5 Protein (Gln19-Cys1676), His-FLAG-tagged+Inquiry
C5-536HRecombinant Human C5 protein, His-tagged+Inquiry
C5-564RRecombinant Rat C5 Protein, His/GST-tagged+Inquiry
C5-092HRecombinant Human C5 protein, Fc-tagged+Inquiry

See All C5 Recombinant Protein

◆ Native Protein

C5-10540HNative Human C5+Inquiry
C5-53HNative Human Complement C5+Inquiry

See All C5 Native Protein

◆ Lysates

C5-8020HCLRecombinant Human C5 293 Cell Lysate+Inquiry

See All C5 Lysates

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