Active Recombinant Cynomolgus FGL1 protein, hFc-tagged, Low Endotoxin

• Cat.No.: FGL1-124C
• Product Overview: Recombinant Cynomolgus FGL1 protein(G7N0K6-1)(23-312 aa), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Protein Length: 23-312 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human LAG3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus FGL1, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 55.5 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.