Recombinant Cynomolgus PVRIG protein, hFc-tagged

• Cat.No.: PVRIG-0606C
• Product Overview: Recombinant Cynomolgus PVRIG protein( A0A2K5WVV8-1)(Thr41-Asp171), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Thr41-Asp171
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.