Recombinant Dengue virus (DENV) (type 2, strain New Guinea C) NS1 protein(Asp1-Ala352), His-tagged

• Cat.No.: NS1-2441D
• Product Overview: Recombinant Dengue virus (type 2, strain New Guinea C) NS1 (AAC59275.1) (Asp1-Ala352) was expressed in HEK293 with a polyhistidine tag at the N-terminus.

Specification

• Species: DENV
• Source: HEK293
• Tag: His
• Protein Length: Asp1-Ala352
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Dengue virus (type 2, strain New Guinea C) NS1 consists of 371 amino acids and predicts a molecular mass of 42.3 kDa. The apparent molecular mass of the protein is approximately 45.8 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.