Recombinant Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E protein(Met578-Gln680), His-tagged

• Cat.No.: E-3337D
• Product Overview: Recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Met578-Gln680) was expressed in Yeast with a polyhistidine tag at the C-terminus.

Specification

• Source: Yeast
• Species: DENV
• Tag: C-His
• Protein length: Met578-Gln680
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 114 amino acids and predicts a molecular mass of 13 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.