Recombinant DENV NS5 protein(Gly2492-Gln2787), His-tagged

• Cat.No.: NS5-359D
• Product Overview: Recombinant Dengue virus (type 2, strain New Guinea C) NS5 (AAC59275.1) (Gly2492-Gln2787) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: DENV
• Source: E.coli
• Tag: His
• Protein Length: Gly2492-Gln2787
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Dengue virus (type 2, strain New Guinea C) NS5 consists 314 amino acids and predicts a molecular mass of 35.5 kDa.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.