"acnB" Related Products

Recombinant E. coli acnB Protein

Cat.No.: acnB-487E
Product Overview: Recombinant E.Coli (strain K12) acnB (P36683) (Met 1-Val 865) was expressed and purified.
Source: E. coli
Species: E. coli
Predicted N Terminal: Met 1
Form: Lyophilized from sterile 40mM Tris, 1mM DTT, pH 8.2, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant E.Coli acnB consisting of 865 amino acids and has a calculated molecular mass of 93.5 kDa. It migrates as an approximately 95 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: acnB aconitate hydratase 2; aconitase B; 2-methyl-cis-aconitate hydratase [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol: acnB
Synonyms: ECK0117; JW0114; yacI; yacJ
Gene ID: 944864
mRNA Refseq:
Protein Refseq: NP_414660
MIM:
UniProt ID:
Pathway: 2-Oxocarboxylic acid metabolism, organism-specific biosystem; Biosynthesis of amino acids, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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