Recombinant E. coli TrpB protein, His-tagged

• Cat.No.: trpB-01E
• Product Overview: Recombinant E. coli TrpB protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-? hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
• Molecular Mass: 28.7&43.8kDa
  28&40-50 kDa
• AA Sequence: Met1-Ser268&Thr2-Ile397
• Endotoxin: < 1.0 EU per μg as determined by the LAL method.
• Purity: > 95%
• Stability: Liquid proteins are stable for up to 6 months when stored at -20 to -80 centigrade.
  Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 centigrade.
• Storage: Store at < -20 centigrade, stable for 6 months. Please minimize freeze-thaw cycles.
• Reconstitution: Please refer to the printed manual for detailed information.
• Handling: Wear appropriate protective clothing and safety gloves. Avoid inhalation. Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure. Mechanical exhaust required. Keep away from ignition sources, heat and flame. Incompatibilities: Strong oxidizing agents. No smoking at working site.
• Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice.Upon receipt, store it immediately at< -20 centigrade.

Gene Information

• Gene Name: trpB tryptophan synthase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: trpB
• Synonyms: Tryptophan synthetase; Tryptophan synthase
• Gene ID: 945768
• Protein Refseq: NP_415777
• UniProt ID: P0A879