Recombinant E. coli zwf Protein zwf-01E

Recombinant E. coli zwf Protein

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Recombinant E. coli zwf Protein

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Cat.No. : zwf-01E
Product Overview : Recombinant E. coli zwf Protein (1-491aa) without tag was expressed in E. coli.
Description : Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
Source : E. coli
Species : E. coli
Form : Liquid
Bio-activity : Specific activity is > 50 unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 centigrade.
Molecular Mass : 55.7 kDa
AA Sequence : MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Purity : > 95% by SDS-PAGE
Applications : Enzyme Activity, SDS-PAGE
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1mg/ml (determined by Bradford assay)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Gene Name : zwf NADP(+)-dependent glucose-6-phosphate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : zwf
Synonyms : zwf; NADP(+)-dependent glucose-6-phosphate dehydrogenase; ECK1853; NADP(+)-dependent glucose-6-phosphate dehydrogenase; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p
Gene ID : 946370
Protein Refseq : NP_416366
UniProt ID : P0AC53

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