Recombinant Escherichia coli gyrA Protein (S2-A523)

• Cat.No.: gyrA-1448E
• Product Overview: Recombinant Escherichia coli (G)-gyrA(S2-A523) protein without tag was expressed in E. coli

Specification

• Description: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to maintain chromosomes in an underwound state. This makes better substrates for topoisomerase IV (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli. Gyrase catalyzes the interconversion of other topological isomers of dsDNA rings, including catenanes. Relaxes negatively supercoiled DNA in an ATP-independent manner (E.coli gyrase has higher supercoiling activity than many other bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli. E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical. The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C-terminal domain (approximately residues 524-841) and specifically the GyrA-box.
• Source: E. coli
• Species: Escherichia coli
• Tag: Tag Free
• Form: Liquid
• Protein Length: S2-A523
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: gyrA DNA gyrase subunit A [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: gyrA
• Synonyms: gyrA; DNA gyrase subunit A; ECK2223; hisW; nalA; nfxA; norA; parD; DNA gyrase subunit A; EC 5.6.2.2
• Gene ID: 946614
• Protein Refseq: NP_416734
• UniProt ID: P0AES4