| Cat.No. : |
MCR1-2371E |
| Product Overview : |
Recombinant Escherichia coli MCR1 Protein (1-541 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length. |
| Description : |
Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A gives polymyxin resistance (PubMed:26603172).1 Publication Confers resistance to polymyxin-type antibiotics; expression of the Mcr-1 protein in E.coli increases colistin and polymyxin B minimal inhibitory concentration (MIC) from 0.5 mg/ml to 2.0 mg/ml. The pHNSHP45 plasmid can transfer efficiently (0.1 to 0.001) to other E.coli strains by conjugation and increases polymxin MIC by 8- to 16-fold; it may not require selective pressure to be maintained in the cell. When transformed into K.pneumoniae or P.aeruginosa it also increases polymxin MIC 8- to 16-fold. In a murine (BALB/c mice) thigh infection study using an mcr1-encoding plasmid isolated from a human patient, the plasmid confers in vivo protection against colistin (PubMed:26603172). |
| Source : |
E. coli |
| Species : |
E. coli |
| Tag : |
His-SUMO |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
76.1 kDa |
| Protein length : |
1-541 aa |
| AA Sequence : |
MMQHTSVWYR RSVSPFVLVA SVAVFLTATA NLTFFDKISQ TYPIADNLGF VLTIAVVLFG AMLLITTLLS SYRYVLKPVL ILLLIMGAVT SYFTDTYGTV YDTTMLQNAL QTDQAETKDL LNAAFIMRII GLGVLPSLLV AFVKVDYPTW GKGLMRRLGL IVASLALILL PVVAFSSHYA SFFRVHKPLR SYVNPIMPIY SVGKLASIEY KKASAPKDTI YHAKDAVQAT KPDMRKPRLV VFVVGETARA DHVSFNGYER DTFPQLAKID GVTNFSNVTS CGTSTAYSVP CMFSYLGADE YDVDTAKYQE NVLDTLDRLG VSILWRDNNS DSKGVMDKLP KAQFADYKSA TNNAICNTNP YNECRDVGML VGLDDFVAAN NGKDMLIMLH QMGNHGPAYF KRYDEKFAKF TPVCEGNELA KCEHQSLINA YDNALLATDD FIAQSIQWLQ THSNAYDVSM LYVSDHGESL GENGVYLHGM PNAFAPKEQR SVPAFFWTDK QTGITPMATD TVLTHDAITP TLLKLFDVTA DKVKDRTAFI R |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA will be sent along with the products. Please refer to it for detailed information. |
| Synonyms : |
mcr1; Polymyxin resistance protein MCR-1; |
| UniProt ID : |
A0A0R6L508 |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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