Recombinant Escherichia coli NRDB Protein (2-376 aa), His-tagged

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Recombinant Escherichia coli NRDB Protein (2-376 aa), His-tagged

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Cat.No. : NRDB-1561E
Product Overview : Recombinant Escherichia coli (strain K12) NRDB Protein (2-376 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description : Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis.
Source : Yeast
Species : E. coli
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 45.4 kDa
Protein length : 2-376 aa
AA Sequence : AYTTFSQTKN DQLKEPMFFG QPVNVARYDQ QKYDIFEKLI EKQLSFFWRP EEVDVSRDRI DYQALPEHEK HIFISNLKYQ TLLDSIQGRS PNVALLPLIS IPELETWVET WAFSETIHSR SYTHIIRNIV NDPSVVFDDI VTNEQIQKRA EGISSYYDEL IEMTSYWHLL GEGTHTVNGK TVTVSLRELK KKLYLCLMSV NALEAIRFYV SFACSFAFAE RELMEGNAKI IRLIARDEAL HLTGTQHMLN LLRSGADDPE MAEIAEECKQ ECYDLFVQAA QQEKDWADYL FRDGSMIGLN KDILCQYVEY ITNIRMQAVG LDLPFQTRSN PIPWINTWLV SDNVQVAPQE VEVSSYLVGQ IDSEVDTDDL SNFQL
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : nrdB ribonucleoside-diphosphate reductase 1 subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : NRDB
Synonyms : ECK2227; ftsB;
Gene ID : 946732
Protein Refseq : NP_416738
UniProt ID : P69924

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