Recombinant Full Length Hepatitis B Surface Ag Protein, His&Avi tagged, Biotinylated

• Cat.No.: HBsAg-02B
• Product Overview: Biotinylated recombinant Full Length Hepatitis B Surface Ag Protein with His&Avi tag was expressed in HEK293.

Specification

• Species: HBV
• Source: HEK293
• Tag: Avi&His
• Storage: Store at -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Stable for 12 months from the date of receipt of the product under proper storage and handling conditions.
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• AA Sequence: MENTTSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGAPTCPGQNSQSPTSNHSPTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLLPGTSTTSTGPCKTCTIPAQGTSMFPSCCCTKPSDGNCTCIPIPSSWAFARFLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYNILSPFLPLLPIFFCLWVYIESGGGGSPGGRKKRRQRRRPQGGGGSGLNDIFEAQKIEWHEHHHHHH
• Endotoxin: <1 EU/μg by LAL
• Purity: > 80% by SDS-PAGE
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Concentration: 0.3 mg/mL
• Conjugation: Biotin

Gene Information

• Synonyms: HBsAg; hepatitis B surface antigen

Reviews

10/13/2025

HBsAg-02B

The protein reagent itself is probably fine but we have a lot of trouble finding the cells of interest. I think our vaccine is very weakly stimulating. We'll keep trying!

Q&As

What size is the recombinant HBsAg? Is it the L, M or S version of the antigen?

The HBsAg proteins are generally provided in 100ug, 500ug, and 1mg packing sizes. We can also aliquot based on your specific requirements. Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. As the sequence of the below antigens is confidential, we cannot tell that if they are L, M, or S versions.