Recombinant Full Length Human AGL Protein, C-Flag-tagged

• Cat.No.: AGL-44HFL
• Product Overview: Recombinant Full Length Human AGL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 174.6 kDa
• AA Sequence: MGHSKQIRILLLNEMEKLEKTLFRL EQGYELQFRLGPTLQGKAVTVYTNY PFPGETFNREKFRSLDWENP TEREDDSDKYCKLNLQQSGSFQYYF LQGNEKSGGGYIVVDPILRVGADNH VLPLDCVTLQTFLAKCLGPF DEWESRLRVAKESGYNMIHFTPLQT LGLSRSCYSLANQLELNPDFSRPNR KYTWNDVGQLVEKLKKEWNV ICITDVVYNHTAANSKWIQEHPECA YNLVNSPHLKPAWVLDRALWRFSCD VAEGKYKEKGIPALIENDHH MNSIRKIIWEDIFPKLKLWEFFQVD VNKAVEQFRRLLTQENRRVTKSDPN QHLTIIQDPEYRRFGCTVDM NIALTTFIPHDKGPAAIEECCNWFH KRMEELNSEKHRLINYHQEQAVNCL LGNVFYERLAGHGPKLGPVT RKHPLVTRYFTFPFEEIDFSMEESM IHLPNKACFLMAHNGWVMGDDPLRN FAEPGSEVYLRRELICWGDS VKLRYGNKPEDCPYLWAHMKKYTEI TATYFQGVRLDNCHSTPLHVAEYML DAARNLQPNLYVVAELFTGS EDLDNVFVTRLGISSLIREAMSAYN SHEEGRLVYRYGGEPVGSFVQPCLR PLMPAIAHALFMDITHDNEC PIVHRSAYDALPSTTIVSMACCASG STRGYDELVPHQISVVSEERFYTKW NPEALPSNTGEVNFQSGIIA ARCAISKLHQELGAKGFIQVYVDQV DEDIVAVTRHSPSIHQSVVAVSRTA FRNPKTSFYSKEVPQMCIPG KIEEVVLEARTIERNTKPYRKDENS INGTPDITVEIREHIQLNESKIVKQ AGVATKGPNEYIQEIEFENL SPGSVIIFRVSLDPHAQVAVGILRN HLTQFSPHFKSGSLAVDNADPILKI PFASLASRLTLAELNQILYR CESEEKEDGGGCYDIPNWSALKYAG LQGLMSVLAEIRPKNDLGHPFCNNL RSGDWMIDYVSNRLISRSGT IAEVGKWLQAMFFYLKQIPRYLIPC YFDAILIGAYTTLLDTAWKQMSSFV QNGSTFVKHLSLGSVQLCGV GKFPSLPILSPALMDVPYRLNEITK EKEQCCVSLAAGLPHFSSGIFRCWG RDTFIALRGILLITGRYVEA RNIILAFAGTLRHGLIPNLLGEGIY ARYNCRDAVWWWLQCIQDYCKMVPN GLDILKCPVSRMYPTDDSAP LPAGTLDQPLFEVIQEAMQKHMQGI QFRERNAGPQIDRNMKDEGFNITAG VDEETGFVYGGNRFNCGTWM DKMGESDRARNRGIPATPRDGSAVE IVGLSKSAVRWLLELSKKNIFPYHE VTVKRHGKAIKVSYDEWNRK IQDNFEKLFHVSEDPSDLNEKHPNL VHKRGIYKDSYGASSPWCDYQLRPN FTIAMVVAPELFTTEKAWKA LEIAEKKLLGPLGMKTLDPDDMVYC GIYDNALDNDNYNLAKGFNYHQGPE WLWPIGYFLRAKLYFSRLMG
  PETTAKTIVLVKNVLSRHYVHLERS PWKGLPELTNENAQYCPFSCETQAW SIATILETLYDLTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism

Gene Information

• Gene Name: AGL amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase [ Homo sapiens (human) ]
• Official Symbol: AGL
• Synonyms: GDE
• Gene ID: 178
• mRNA Refseq: NM_000642.3
• Protein Refseq: NP_000633.2
• MIM: 610860
• UniProt ID: P35573