Recombinant Full Length Human ALKBH8 Protein, C-Flag-tagged

• Cat.No.: ALKBH8-1952HFL
• Product Overview: Recombinant Full Length Human ALKBH8 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables tRNA (uracil) methyltransferase activity; tRNA binding activity; and zinc ion binding activity. Involved in cellular response to DNA damage stimulus; tRNA methylation; and tRNA wobble uridine modification. Located in cytosol and nuclear body. Implicated in autosomal recessive non-syndromic intellectual disability.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 75 kDa
• AA Sequence: MDSNHQSNYKLSKTEKKFLRKQIKA KHTLLRHEGIETVSYATQSLVVANG GLGNGVSRNQLLPVLEKCGL VDALLMPPNKPYSFARYRTTEESKR AYVTLNGKEVVDDLGQKITLYLNFV EKVQWKELRPQALPPGLMVV EEIISSEEEKMLLESVDWTEDTDNQ NSQKSLKHRRVKHFGYEFHYENNNV DKDKPLSGGLPDICESFLEK WLRKGYIKHKPDQMTINQYEPGQGI PAHIDTHSAFEDEIVSLSLGSEIVM DFKHPDGIAVPVMLPRRSLL VMTGESRYLWTHGITCRKFDTVQAS ESLKSGIITSDVGDLTLSKRGLRTS FTFRKVRQTPCNCSYPLVCD SQRKETPPSFPESDKEASRLEQEYV HQVYEEIAGHFSSTRHTPWPHIVEF LKALPSGSIVADIGCGNGKY LGINKELYMIGCDRSQNLVDICRER QFQAFVCDALAVPVRSGSCDACISI AVIHHFATAERRVAALQEIV RLLRPGGKALIYVWAMEQEYNKQKS KYLRGNRNSQGKKEEMNSDTSVQRS LVEQMRDMGSRDSASSVPRI NDSQEGGCNSRQVSNSKLPVHVNRT SFYSQDVLVPWHLKGNPDKGKPVEP FGPIGSQDPSPVFHRYYHVF REGELEGACRTVSDVRILQSYYDQG NWCVILQKA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: ALKBH8 alkB homolog 8, tRNA methyltransferase [ Homo sapiens (human) ]
• Official Symbol: ALKBH8
• Synonyms: ABH8; TRM9; MRT71; TRMT9; TRMT9A
• Gene ID: 91801
• mRNA Refseq: NM_138775.3
• Protein Refseq: NP_620130
• MIM: 613306
• UniProt ID: Q96BT7