Recombinant Full Length Human ARFGAP1 Protein, C-Flag-tagged

• Cat.No.: ARFGAP1-1445HFL
• Product Overview: Recombinant Full Length Human ARFGAP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 45.5 kDa
• AA Sequence: MASPRTRKVLKEVRVQDENNVCFEC GAFNPQWVSVTYGIWICLECSGRHR GLGVHLSFVRSVTMDKWKDI ELEKMKAGGNAKFREFLESQEDYDP CWSLQEKYNSRAAALFRDKVVALAE GREWSLESSPAQNWTPPQPR TLPSMVHRVSGQPQSVTASSDKAFE DWLNDDLGSYQGAQGNRYVGFGNTP PPQKKEDDFLNNAMSSLYSG WSSFTTGASRFASAAKEGATKFGSQ ASQKFWGHKQQPEPASELGHSLNEN VLKPAQEKVKEGKIFDDVSS GVSQLASKGVGSKGWRDVTTFFSGK AEGPLDSPSEGHSYQNSGLDHFQNS NIDQSFWETFGSAEPTKTRK
  SPSSDSWTCADTSTERRSSDSWEVW GSASTNRNSNSDGGEGGEGTKKAVP PAVPTDDGWDNQNWTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Endocytosis

Gene Information

• Gene Name: ARFGAP1 ADP ribosylation factor GTPase activating protein 1 [ Homo sapiens (human) ]
• Official Symbol: ARFGAP1
• Synonyms: ARF1GAP; HRIHFB2281
• Gene ID: 55738
• mRNA Refseq: NM_175609.3
• Protein Refseq: NP_783202.1
• MIM: 608377
• UniProt ID: Q8N6T3