| Description : |
This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. [provided by RefSeq |
| Source : |
In Vitro Cell Free System |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
111.87 kDa |
| Protein Length : |
777 amino acids |
| AA Sequence : |
MPDNRQPRNRQPRIRSGNEPRSAPA MEPDGRGAWAHSRAALDRLEKLLRC SRCTNILREPVCLGGCEHIFCSNCV SDCIGTGCPVCYTPAWIQDLKINRQ LDSMIQLCSKLRNLLHDNELSDLKE DKPRKSLFNDAGSKKNSIKMWFSPR SKKVRYVVSKASVQTQPAIKKDASA QQDSYEFVSPSPPADVSERAKKASA RSGKKQKKKTLAEINQKWNLEAEKE DGEFDSKEESKQKLVSFCSQPSVIS SPQINGEIDLLASGSLTESECFGSL TEVSLPLAEQIESPDTKSRNEVVTP EKVCKNYLTSKKSLPLENNGKRGHH NRLSSPISKRCRTSILSTSGDFVKQ TVPSENIPLPECSSPPSCKRKVGGT SGSKNSNMSDEFISLSPGTPPSTLS SSSYRRVMSSPSAMKLLPNMAVKRN HRGETLLHIASIKGDIPSVEYLLQN GSDPNVKDHAGWTPLHEACNHGHLK VVELLLQHKALVNTTGYQNDSPLHD AAKNGHMDIVKLLLSYGASRNAVNI FGLRPVDYTDDESMKSLLLLPEKNE SSSASHCSVMNTGQRRDGPLVLIGS GLSSEQQKMLSELAVILKAKKYTEF DSTVTHVVVPGDAVQSTLKCMLGIL NGCWILKFEWVKACLRRKVCEQEEK YEIPEGPRRSRLNREQLLPKLFDGC YFYLWGTFKHHPKDNLIKLVTAGGG QILSRKPKPDSDVTQTINTVAYHAR PDSDQRFCTQYIIYEDLCNYHPERV RQGKVWKAPSSWFIDCVMSFELLPL DS |
| Notes : |
Best use within three months from the date of receipt of this protein. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
