Recombinant Full Length Human BUB1 Protein, C-Flag-tagged

• Cat.No.: BUB1-1746HFL
• Product Overview: Recombinant Full Length Human BUB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 122.2 kDa
• AA Sequence: MDTPENVLQMLEAHMQSYKGNDPLG EWERYIQWVEENFPENKEYLITLLE HLMKEFLDKKKYHNDPRFIS YCLKFAEYNSDLHQFFEFLYNHGIG TLSSPLYIAWAGHLEAQGELQHASA VLQRGIQNQAEPREFLQQQY RLFQTRLTETHLPAQARTSEPLHNV QVLNQMITSKSNPGNNMACISKNQG SELSGVISSACDKESNMERR VITISKSEYSVHSSLASKVDVEQVV MYCKEKLIRGESEFSFEELRAQKYN QRRKHEQWVNEDRHYMKRKE ANAFEEQLLKQKMDELHKKLHQVVE TSHEDLPASQERSEVNPARMGPSVG SQQELRAPCLPVTYQQTPVN MEKNPREAPPVVPPLANAISAALVS PATSQSIAPPVPLKAQTVTDSMFAV ASKDAGCVNKSTHEFKPQSG AEIKEGCETHKVANTSSFHTTPNTS LGMVQATPSKVQPSPTVHTKEALGF IMNMFQAPTLPDISDDKDEW QSLDQNEDAFEAQFQKNVRSSGAWG VNKIISSLSSAFHVFEDGNKENYGL PQPKNKPTGARTFGERSVSR LPSKPKEEVPHAEEFLDDSTVWGIR CNKTLAPSPKSPGDFTSAAQLASTP FHKLPVESVHILEDKENVVA KQCTQATLDSCEENMVVPSRDGKFS PIQEKSPKQALSSHMYSASLLRLSQ PAAGGVLTCEAELGVEACRL TDTDAAIAEDPPDAIAGLQAEWMQM SSLGTVDAPNFIVGNPWDDKLIFKL LSGLSKPVSSYPNTFEWQCK LPAIKPKTEFQLGSKLVYVHHLLGE GAFAQVYEATQGDLNDAKNKQKFVL KVQKPANPWEFYIGTQLMER LKPSMQHMFMKFYSAHLFQNGSVLV GELYSYGTLLNAINLYKNTPEKVMP QGLVISFAMRMLYMIEQVHD CEIIHGDIKPDNFILGNGFLEQDDE DDLSAGLALIDLGQSIDMKLFPKGT IFTAKCETSGFQCVEMLSNK PWNYQIDYFGVAATVYCMLFGTYMK VKNEGGECKPEGLFRRLPHLDMWNE FFHVMLNIPDCHHLPSLDLL
  RQKLKKVFQQHYTNKIRALRNRLIV LLLECKRSRKTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: BUB1 BUB1 mitotic checkpoint serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: BUB1
• Synonyms: BUB1A; BUB1L; hBUB1; MCPH30
• Gene ID: 699
• mRNA Refseq: NM_004336.5
• Protein Refseq: NP_004327.1
• MIM: 602452
• UniProt ID: O43683