Recombinant Full Length Human CYLD Protein, C-Flag-tagged

• Cat.No.: CYLD-943HFL
• Product Overview: Recombinant Full Length Human CYLD Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 106.9 kDa
• AA Sequence: MSSGLWSQEKVTSPYWEERIFYLLL QECSVTDKQTQKLLKVPKGSIGQYI QDRSVGHSRIPSAKGKKNQI GLKILEQPHAVLFVDEKDVVEINEK FTELLLAITNCEERFSLFKNRNRLS KGLQIDVGCPVKVQLRSGEE KFPGVVRFRGPLLAERTVSGIFFGV ELLEEGRGQGFTDGVYQGKQLFQCD EDCGVFVALDKLELIEDDDT ALESDYAGPGDTMQVELPPLEINSR VSLKVGETIESGTVIFCDVLPGKES LGYFVGVDMDNPIGNWDGRF DGVQLCSFACVESTILLHINDIIPE SVTQERRPPKLAFMSRGVGDKGSSS HNKPKATGSTSDPGNRNRSE LFYTLNGSSVDSQPQSKSKNTWYID EVAEDPAKSLTEISTDFDRSSPPLQ PPPVNSLTTENRFHSLPFSL TKMPNTNGSIGHSPLSLSAQSVMEE LNTAPVQESPPLAMPPGNSHGLEVG SLAEVKENPPFYGVIRWIGQ PPGLNEVLAGLELEDECAGCTDGTF RGTRYFTCALKKALFVKLKSCRPDS RFASLQPVSNQIERCNSLAF GGYLSEVVEENTPPKMEKEGLEIMI GKKKGIQGHYNSCYLDSTLFCLFAF SSVLDTVLLRPKEKNDVEYY SETQELLRTEIVNPLRIYGYVCATK IMKLRKILEKVEAASGFTSEEKDPE EFLNILFHHILRVEPLLKIR SAGQKVQDCYFYQIFMEKNEKVGVP TIQQLLEWSFINSNLKFAEAPSCLI IQMPRFGKDFKLFKKIFPSL ELNITDLLEDTPRQCRICGGLAMYE CRECYDDPDISAGKIKQFCKTCNTQ VHLHPKRLNHKYNPVSLPKD LPDWDWRHGCIPCQNMELFAVLCIE TSHYVAFVKYGKDDSAWLFFDSMAD RDGGQNGFNIPQVTPCPEVG
  EYLKMSLEDLHSLDSRRIQGCARRL LCDAYMCMYQSPTMSLYKTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway

Gene Information

• Gene Name: CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ]
• Official Symbol: CYLD
• Synonyms: EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; FTDALS8
• Gene ID: 1540
• mRNA Refseq: NM_001042355.2
• Protein Refseq: NP_001035814.1
• MIM: 605018
• UniProt ID: Q9NQC7