Recombinant Full Length Human CYP17A1 Protein, C-Flag-tagged

• Cat.No.: CYP17A1-795HFL
• Product Overview: Recombinant Full Length Human CYP17A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.2 kDa
• AA Sequence: MWELVALLLLTLAYLFWPKRRCPGA KYPKSLLSLPLVGSLPFLPRHGHMH NNFFKLQKKYGPIYSVRMGT KTTVIVGHHQLAKEVLIKKGKDFSG RPQMATLDIASNNRKGIAFADSGAH WQLHRRLAMATFALFKDGDQ KLEKIICQEISTLCDMLATHNGQSI DISFPVFVAVTNVISLICFNTSYKN GDPELNVIQNYNEGIIDNLS KDSLVDLVPWLKIFPNKTLEKLKSH VKIRNDLLNKILENYKEKFRSDSIT NMLDTLMQAKMNSDNGNAGP DQDSELLSDNHILTTIGDIFGAGVE TTTSVVKWTLAFLLHNPQVKKKLYE EIDQNVGFSRTPTISDRNRL LLLEATIREVLRLRPVAPMLIPHKA NVDSSIGEFAVDKGTEVIINLWALH HNEKEWHQPDQFMPERFLNP AGTQLISPSVSYLPFGAGPRSCIGE ILARQELFLIMAWLLQRFDLEVPDD GQLPSLEGIPKVVFLIDSFK
  VKIKVRQAWREAQAEGSTTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways

Gene Information

• Gene Name: CYP17A1 cytochrome P450 family 17 subfamily A member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP17A1
• Synonyms: CPT7; CYP17; S17AH; P450C17
• Gene ID: 1586
• mRNA Refseq: NM_000102.4
• Protein Refseq: NP_000093.1
• MIM: 609300
• UniProt ID: P05093