Recombinant Full Length Human DAAM1 Protein, C-Flag-tagged

• Cat.No.: DAAM1-1134HFL
• Product Overview: Recombinant Full Length Human DAAM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 123.3 kDa
• AA Sequence: MAPRKRGGRGISFIFCCFRNNDHPE ITYRLRNDSNFALQTMEPALPMPPV EELDVMFSELVDELDLTDKH REAMFALPAEKKWQIYCSKKKDQEE NKGATSWPEFYIDQLNSMAARKSLL ALEKEEEEERSKTIESLKTA LRTKPMRFVTRFIDLDGLSCILNFL KTMDYETSESRIHTSLIGCIKALMN NSQGRAHVLAHSESINVIAQ SLSTENIKTKVAVLEILGAVCLVPG GHKKVLQAMLHYQKYASERTRFQTL INDLDKSTGRYRDEVSLKTA IMSFINAVLSQGAGVESLDFRLHLR YEFLMLGIQPVIDKLREHENSTLDR HLDFFEMLRNEDELEFAKRF ELVHIDTKSATQMFELTRKRLTHSE AYPHFMSILHHCLQMPYKRSGNTVQ YWLLLDRIIQQIVIQNDKGQ DPDSTPLENFNIKNVVRMLVNENEV KQWKEQAEKMRKEHNELQQKLEKKE RECDAKTQEKEEMMQTLNKM KEKLEKETTEHKQVKQQVADLTAQL HELSRRAVCASIPGGPSPGAPGGPF PSSVPGSLLPPPPPPPLPGG MLPPPPPPLPPGGPPPPPGPPPLGA IMPPPGAPMGLALKKKSIPQPTNAL KSFNWSKLPENKLEGTVWTE IDDTKVFKILDLEDLERTFSAYQRQ QDFFVNSNSKQKEADAIDDTLSSKL KVKELSVIDGRRAQNCNILL SRLKLSNDEIKRAILTMDEQEDLPK DMLEQLLKFVPEKSDIDLLEEHKHE LDRMAKADRFLFEMSRINHY QQRLQSLYFKKKFAERVAEVKPKVE AIRSGSEEVFRSGALKQLLEVVLAF GNYMNKGQRGNAYGFKISSL NKIADTKSSIDKNITLLHYLITIVE NKYPSVLNLNEELRDIPQAAKVNMT ELDKEISTLRSGLKAVETEL EYQKSQPPQPGDKFVSVVSQFITVA SFSFSDVEDLLAEAKDLFTKAVKHF GEEAGKIQPDEFFGIFDQFL QAVSEAKQENENMRKKKEEEERRAR MEAQLKEQRERERKMRKAKENSEES GEFDDLVSALRSGEVFDKDL
  SKLKRNRKRITNQMTDSSRERPITK LNFTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Wnt signaling pathway

Gene Information

• Gene Name: DAAM1 dishevelled associated activator of morphogenesis 1 [ Homo sapiens (human) ]
• Official Symbol: DAAM1
• Synonyms: FLJ41657; KIAA0666
• Gene ID: 23002
• mRNA Refseq: NM_014992.2
• Protein Refseq: NP_055807.1
• MIM: 606626
• UniProt ID: Q9Y4D1