Recombinant Full Length Human DGKZ Protein, C-Flag-tagged

• Cat.No.: DGKZ-542HFL
• Product Overview: Recombinant Full Length Human DGKZ Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 103.9 kDa
• AA Sequence: MEPRDGSPEARSSDSESASASSSGS ERDAGPEPDKAPRRLNKRRFPGLRL FGHRKAITKSGLQHLAPPPP TPGAPCSESERQIRSTVDWSESATY GEHIWFETNVSGDFCYVGEQYCVAR MLQKSVSRRKCAACKIVVHT PCIEQLEKINFRCKPSFRESGSRNV REPTFVRHHWVHRRRQDGKCRHCGK GFQQKFTFHSKEIVAISCSW CKQAYHSKVSCFMLQQIEEPCSLGV HAAVVIPPTWILRARRPQNTLKASK KKKRASFKRKSSKKGPEEGR WRPFIIRPTPSPLMKPLLVFVNPKS GGNQGAKIIQSFLWYLNPRQVFDLS QGGPKEALEMYRKVHNLRIL ACGGDGTVGWILSTLDQLRLKPPPP VAILPLGTGNDLARTLNWGGGYTDE PVSKILSHVEEGNVVQLDRW DLHAEPNPEAGPEDRDEGATDRLPL DVFNNYFSLGFDAHVTLEFHESREA NPEKFNSRFRNKMFYAGTAF SDFLMGSSKDLAKHIRVVCDGMDLT PKIQDLKPKCVVFLNIPRYCAGTMP WGHPGEHHDFEPQRHDDGYL EVIGFTMTSLAALQVGGHGERLTQC REVVLTTSKAIPVQVDGEPCKLAAS RIRIALRNQATMVQKAKRRS AAPLHSDQQPVPEQLRIQVSRVSMH DYEALHYDKEQLKEASVPLGTVVVP GDSDLELCRAHIERLQQEPD GAGAKSPTCQKLSPKWCFLDATTAS RFYRIDRAQEHLNYVTEIAQDEIYI LDPELLGASARPDLPTPTSP LPTSPCSPTPRSLQGDAAPPQGEEL IEAAKRNDFCKLQELHRAGGDLMHR DEQSRTLLHHAVSTGSKDVV RYLLDHAPPEILDAVEENGETCLHQ AAALGQRTICHYIVEAGASLMKTDQ QGDTPRQRAEKAQDTELAAY
  LENRQHYQMIQREDQETAVTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system

Gene Information

• Gene Name: DGKZ diacylglycerol kinase zeta [ Homo sapiens (human) ]
• Official Symbol: DGKZ
• Synonyms: DAGK5; DAGK6; DGK-ZETA; hDGKzeta
• Gene ID: 8525
• mRNA Refseq: NM_003646.4
• Protein Refseq: NP_003637.2
• MIM: 601441
• UniProt ID: Q13574