Recombinant Full Length Human EHHADH Protein, C-Flag-tagged

• Cat.No.: EHHADH-1458HFL
• Product Overview: Recombinant Full Length Human EHHADH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 79.3 kDa
• AA Sequence: MAEYTRLHNALALIRLRNPPVNAIS TTLLRDIKEGLQKAVIDHTIKAIVI CGAEGKFSAGADIRGFSAPR TFGLTLGHVVDEIQRNEKPVVAAIQ GMAFGGGLELALGCHYRIAHAEAQV GLPEVTLGLLPGARGTQLLP RLTGVPAALDLITSGRRILADEALK LGILDKVVNSDPVEEAIRFAQRVSD QPLESRRLCNKPIQSLPNMD SIFSEALLKMRRQHPGCLAQEACVR AVQAAVQYPYEVGIKKEEELFLYLL QSGQARALQYAFFAERKANK WSTPSGASWKTASARPVSSVGVVGL GTMGRGIVISFARARIPVIAVDSDK NQLATANKMITSVLEKEASK MQQSGHPWSGPKPRLTSSVKELGGV DLVIEAVFEEMSLKKQVFAELSAVC KPEAFLCTNTSALDVDEIAS STDRPHLVIGTHFFSPAHVMKLLEV IPSQYSSPTTIATVMNLSKKIKKIG VVVGNCFGFVGNRMLNPYYN QAYFLLEEGSKPEEVDQVLEEFGFK MGPFRVSDLAGLDVGWKSRKGQGLT GPTLLPGTPARKRGNRRYCP IPDVLCELGRFGQKTGKGWYQYDKP LGRIHKPDPWLSKFLSRYRKTHHIE PRTISQDEILERCLYSLINE AFRILGEGIAASPEHIDVVYLHGYG WPRHKGGPMFYASTVGLPTVLEKLQ KYYRQNPDIPQLEPSDYLKK
  LASQGNPPLKEWQSLAGSPSSKLTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: EHHADH enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: EHHADH
• Synonyms: LBP; ECHD; LBFP; MFE1; PBFE; FRTS3; L-PBE
• Gene ID: 1962
• mRNA Refseq: NM_001966.4
• Protein Refseq: NP_001957.2
• MIM: 607037
• UniProt ID: Q08426