Recombinant Full Length Human GAK Protein, C-Flag-tagged

• Cat.No.: GAK-1336HFL
• Product Overview: Recombinant Full Length Human GAK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 143 kDa
• AA Sequence: MSLLQSALDFLAGPGSLGGASGRDQ SDFVGQTVELGELRLRVRRVLAEGG FAFVYEAQDVGSGREYALKR LLSNEEEKNRAIIQEVCFMKKLSGH PNIVQFCSAASIGKEESDTGQAEFL LLTELCKGQLVEFLKKMESR GPLSCDTVLKIFYQTCRAVQHMHRQ KPPIIHRDLKVENLLLSNQGTIKLC DFGSATTISHYPDYSWSAQR RALVEEEITRNTTPMYRTPEIIDLY SNFPIGEKQDIWALGCILYLLCFRQ HPFEDGAKLRIVNGKYSIPP HDTQYTVFHSLIRAMLQVNPEERLS IAEVVHQLQEIAAARNVNPKSPITE LLEQNGGYGSATLSRGPPPP VGPAGSGYSGGLALAEYDQPYGGFL DILRGGTERLFTNLKDTSSKVIQSV ANYAKGDLDISYITSRIAVM SFPAEGVESALKNNIEDVRLFLDSK HPGHYAVYNLSPRTYRPSRFHNRVS ECGWAARRAPHLHTLYNICR NMHAWLRQDHKNVCVVHCMDGRAAS AVAVCSFLCFCRLFSTAEAAVYMFS MKRCPPGIWPSHKRYIEYMC DMVAEEPITPHSKPILVRAVVMTPV PLFSKQRSGCRPFCEVYVGDERVAS TSQEYDKMRDFKIEDGKAVI PLGVTVQGDVLIVIYHARSTLGGRL QAKMASMKMFQIQFHTGFVPRNATT VKFAKYDLDACDIQEKYPDL FQVNLEVEVEPRDRPSREAPPWENS SMRGLNPKILFSSREEQQDILSKFG KPELPRQPGSTAQYDAGAGS PEAEPTDSDSPPSSSADASRFLHTL DWQEEKEAETGAENASSKESESALM EDRDESEVSDEGGSPISSEG QEPRADPEPPGLAAGLVQQDLVFEV ETPAVLPEPVPQEDGVDLLGLHSEV GAGPAVPPQACKAPSSNTDL LSCLLGPPEAASQGPPEDLLSEDPL LLASPAPPLSVQSTPRGGPPAAADP FGPLLPSSGNNSQPCSNPDL FGEFLNSDSVTVPPSFPSAHSAPPP SCSADFLHLGDLPGEPSKMTASSSN PDLLGGWAAWTETAASAVAP TPATEGPLFSPGGQPAPCGSQASWT KSQNPDPFADLGDLSSGLQGSPAGF PPGGFIPKTATTPKGSSSWQ TSRPPAQGASWPPQAKPPPKACTQP RPNYASNFSVIGAREERGVRAPSFA QKPKVSENDFEDLLSNQGFS SRSDKKGPKTIAEMRKQDLAKDTDP LKLKLLDWIEGKERNIRALLSTLHT VLWDGESRWTPVGMADLVAP
  EQVKKHYRRAVLAVHPDKAAGQPYE QHAKMIFMELNDAWSEFENQGSRPL FSGPTRTRRLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: GAK cyclin G associated kinase [ Homo sapiens (human) ]
• Official Symbol: GAK
• Synonyms: DNAJ26; DNAJC26
• Gene ID: 2580
• mRNA Refseq: NM_005255.4
• Protein Refseq: NP_005246.2
• MIM: 602052
• UniProt ID: O14976