Recombinant Full Length Human HSPA8 Protein, C-Flag-tagged

• Cat.No.: HSPA8-342HFL
• Product Overview: Recombinant Full Length Human HSPA8 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.7 kDa
• AA Sequence: MSKGPAVGIDLGTTYSCVGVFQHGK VEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVAMNPTNTVFDA KRLIGRRFDDAVVQSDMKHWPFMVV NDAGRPKVQVEYKGETKSFYPEEVS SMVLTKMKEIAEAYLGKTVT NAVVTVPAYFNDSQRQATKDAGTIA GLNVLRIINEPTAAAIAYGLDKKVG AERNVLIFDLGGGTFDVSIL TIEDGIFEVKSTAGDTHLGGEDFDN RMVNHFIAEFKRKHKKDISENKRAV RRLRTACERAKRTLSSSTQA SIEIDSLYEGIDFYTSITRARFEEL NADLFRGTLDPVEKALRDAKLDKSQ IHDIVLVGGSTRIPKIQKLL QDFFNGKELNKSINPDEAVAYGAAV QAAILSGDKSENVQDLLLLDVTPLS LGIETAGGVMTVLIKRNTTI PTKQTQTFTTYSDNQPGVLIQVYEG ERAMTKDNNLLGKFELTGIPPAPRG VPQIEVTFDIDANGILNVSA VDKSTGKENKITITNDKGRLSKEDI ERMVQEAEKYKAEDEKQRDKVSSKN SLESYAFNMKATVEDEKLQG KINDEDKQKILDKCNEIINWLDKNQ TAEKEEFEHQQKELEKVCNPIITKL YQSAGGMPGGMPGGFPGGGA
  PPSGGASSGPTIEEVDTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome

Gene Information

• Gene Name: HSPA8 heat shock protein family A (Hsp70) member 8 [ Homo sapiens (human) ]
• Official Symbol: HSPA8
• Synonyms: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• Gene ID: 3312
• mRNA Refseq: NM_006597.6
• Protein Refseq: NP_006588.1
• MIM: 600816
• UniProt ID: P11142