Recombinant Full Length Human INPP5B Protein, GST-tagged

• Cat.No.: INPP5B-5954HF
• Product Overview: Human INPP5B full-length ORF ( AAH58932.1, 1 a.a. - 748 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). This gene encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 111.4 kDa
• Protein length: 748 amino acids
• AA Sequence: MDQSVAIQETLAEGEYCVIAVQGVL CEGDSRQSRLLGLVRYRLEHGGQEH ALFLYTHRRMAITGDDVSLDQIVPV SRDFTLEEVSPDGELYILGSDVTVQ LDTAELSLVFQLPFGSQTRMFLHEV ARACPGFDSATRDPEFLWLSRYRCA ELELEMPTPRGCNSALVTWPGYATI GGGGSNFDGLRPNGKGVPMDQSSRG QDKPESLQPRQNKSKSEITDMVRSS TITVSDKAHILSMQKFGLRDTIVKS HLLQKEEDYTYIQNFRFFAGTYNVN GQSPKECLRLWLSNGIQAPDVYCVG FQELDLSKEAFFFHDTPKEEEWFKA VSEGLHPDAKYAKVKLIRLVGIMLL LYVKQEHAAYISEVEAETVGTGIMG RMGNKGGVAIRFQFHNTSICVVNSH LAAHIEEYERRNQDYKDICSRMQFC QPDPSLPPLTISNHDVILWLGDLNY RIEELDVEKVKKLIEEKDFQMLYAY DQLKIQVAAKTVFEGFTEGELTFQP TYKYDTGSDDWDTSEKCRAPAWCDR ILWKGKNITQLSYQSHMALKTSDHK PVSSVFDIGVRVVNDELYRKTLEEI VRSLDKMENANIPSVSLSKREFCFQ NVKYMQLKVESFTIHNGQVPCHFEF INKPDEESYCKQWLNANPSRGFLLP DSDVEIDLELFVNKTTATKLNSGED KIEDILVLHLDRGKDYFLSVSGNYL PSCFGSPIHTLCYMREPILDLPLET ISELLAYLAAYCFETQLVTKSLI
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: INPP5B inositol polyphosphate-5-phosphatase, 75kDa [ Homo sapiens ]
• Official Symbol: INPP5B
• Synonyms: INPP5B; inositol polyphosphate-5-phosphatase, 75kDa; inositol polyphosphate 5 phosphatase, 75kD; type II inositol 1,4,5-trisphosphate 5-phosphatase; phosphoinositide 5-phosphatase; 75 kDa inositol polyphosphate-5-phosphatase; type II inositol-1,4,5-trisphosphate 5-phosphatase; 5PTase; MGC65156; MGC71303
• Gene ID: 3633
• mRNA Refseq: NM_005540
• Protein Refseq: NP_005531
• MIM: 147264
• UniProt ID: P32019

Reviews

02/15/2020

We found the product's straightforward protocols highly conducive to seamless integration into our lab work.

10/17/2017

Clear and concise instructions simplified the application of the product, reducing the learning curve.

06/18/2017

We appreciated the product's efficiency in achieving target protein upregulation with minimal time investment.

Q&As

What is the subcellular localization of INPP5B?

INPP5B is primarily located in the cytoplasm, where it functions to regulate inositol phosphate levels within the cell.

How is INPP5B activity regulated?

The activity of INPP5B can be regulated through post-translational modifications and interactions with other proteins.

What is the role of INPP5B in cellular signaling?

INPP5B regulates cellular signaling by controlling the levels of inositol phosphates, which are essential secondary messengers in various cellular pathways.

Is there any research on INPP5B as a therapeutic target?

INPP5B is a subject of research in cancer therapy, as its modulation may affect the behavior of cancer cells.

What is INPP5B?

INPP5B is an enzyme involved in the dephosphorylation of inositol phosphates, specifically in the conversion of inositol 1,4,5-trisphosphate (IP3) to inositol 1,4-bisphosphate.

In which cellular processes does INPP5B play a role?

INPP5B is involved in regulating processes such as cell growth, proliferation, and cytoskeletal dynamics through its influence on inositol phosphate signaling.

What are the implications of INPP5B dysregulation?

Dysregulation of INPP5B can impact various cellular functions and has been associated with conditions like cancer, as it can alter signaling pathways that control cell growth and survival.