Recombinant Full Length Human LPIN1 Protein, C-Flag-tagged

• Cat.No.: LPIN1-1448HFL
• Product Overview: Recombinant Full Length Human LPIN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 98.5 kDa
• AA Sequence: MNYVGQLAGQVFVTVKELYKGLNPA TLSGCIDIIVIRQPNGNLQCSPFHV RFGKMGVLRSREKVVDIEIN GESVDLHMKLGDNGEAFFVQETDND QEVIPMHLATSPILSEGASRMECQL KRGSVDRMRGLDPSTPAQVI APSETPSSSSVVKKRRKRRRKSQLD SLKRDDNMNTSEDEDMFPIEMSSDE AMELLESSRTLPNDIPPFQD DIPEENLSLAVIYPQSASYPNSDRE WSPTPSPSGSRPSTPKSDSELVSKS TERTGQKNPEMLWLWGELPQ AAKSSSPHKMKESSPLSSRKICDKS HFQAIHSESSDTFSDQSPTLVGGAL LDQNKPQTEMQFVNEEDLET LGAAAPLLPMIEELKPPSASVVQTA NKTDSPSRKRDKRSRHLGADGVYLD DLTDMDPEVAALYFPKNGDP SGLAKHASDNGARSANQSPQSVGSS GVDSGVESTSDGLRDLPSIAISLCG GLSDHREITKDAFLEQAVSY QQFVDNPAIIDDPNLVVKIGSKYYN WTTAAPLLLAMQAFQKPLPKATVES IMRDKMPKKGGRWWFSWRGR NTTIKEESKPEQCLAGKAHSTGEQP PQLSLATRVKHESSSSDEERAAAKP SNAGHLPLLPNVSYKKTLRL TSEQLKSLKLKNGPNDVVFSVTTQY QGTCRCEGTIYLWNWDDKVIISDID GTITRSDTLGHILPTLGKDW THQGIAKLYHKVSQNGYKFLYCSAR AIGMADMTRGYLHWVNERGTVLPQG PLLLSPSSLFSALHREVIEK KPEKFKVQCLTDIKNLFFPNTEPFY AAFGNRPADVYSYKQVGVSLNRIFT VNPKGELVQEHAKTNISSYV
  RLCEVVDHVFPLLKRSHSSDFPCSD TFSNFTFWREPLPPFENQDIHSASA TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: LPIN1 lipin 1 [ Homo sapiens (human) ]
• Official Symbol: LPIN1
• Synonyms: PAP1
• Gene ID: 23175
• mRNA Refseq: NM_145693.4
• Protein Refseq: NP_663731.1
• MIM: 605518
• UniProt ID: Q14693