Recombinant Full Length Human NMT1 Protein, C-Flag-tagged

• Cat.No.: NMT1-1355HFL
• Product Overview: Recombinant Full Length Human NMT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.6 kDa
• AA Sequence: MADESETAVKPPAPPLPQMMEGNGN GHEHCSDCENEEDNSYNRGGLSPAN DTGAKKKKKKQKKKKEKGSE TDSAQDQPVKMNSLPAERIQEIQKA IELFSVGQGPAKTMEEASKRSYQFW DTQPVPKLGEVVNTHGPVEP DKDNIRQEPYTLPQGFTWDALDLGD RGVLKELYTLLNENYVEDDDNMFRF DYSPEFLLWALRPPGWLPQW HCGVRVVSSRKLVGFISAIPANIHI YDTEKKMVEINFLCVHKKLRSKRVA PVLIREITRRVHLEGIFQAV YTAGVVLPKPVGTCRYWHRSLNPRK LIEVKFSHLSRNMTMQRTMKLYRLP ETPKTAGLRPMETKDIPVVH QLLTRYLKQFHLTPVMSQEEVEHWF YPQENIIDTFVVENANGEVTDFLSF YTLPSTIMNHPTHKSLKAAY SFYNVHTQTPLLDLMSDALVLAKMK GFDVFNALDLMENKTFLEKLKFGIG DGNLQYYLYNWKCPSMGAEK
  VGLVLQTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: NMT1 N-myristoyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: NMT1
• Synonyms: NMT; HsNMT1
• Gene ID: 4836
• mRNA Refseq: NM_021079.5
• Protein Refseq: NP_066565.1
• MIM: 160993
• UniProt ID: P30419