Recombinant Full Length Human NOS2 Protein, C-Flag-tagged

• Cat.No.: NOS2-986HFL
• Product Overview: Recombinant Full Length Human NOS2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 130.9 kDa
• AA Sequence: MACPWKFLFKTKFHQYAMNGEKDIN NNVEKAPCATSSPVTQDDLQYHNLS KQQNESPQPLVETGKKSPES LVKLDATPLSSPRHVRIKNWGSGMT FQDTLHHKAKGILTCRSKSCLGSIM TPKSLTRGPRDKPTPPDELL PQAIEFVNKYYGSFKEAKIEEHLAR VEAVTKEIETTGTYQLTGDELIFAT KQAWRNAPRCIGRIQWSNLQ VFDARSCSTAREMFEHICRHVRYST NNGNIRSAITVFPQRSDGKHDFRVW NAQLIRYAGYQMPDGSIRGD PANVEFTQLCIDLGWKPKYGRFDVV PLVLQANGRDPELFEIPPDLVLEVA MEHPKYEWFRELELKWYALP AVANMLLEVGGLEFPGCPFNGWYMG TEIGVRDFCDVQRYNILEEVGRRMG LETHKLASLWKDQAVVEINI AVLHSFQKQNVTIMDHHSAAESFMK YMQNEYRSRGGCPADWIWLVPPMSG SITPVFHQEMLNYVLSPFYY YQVEAWKTHVWQDEKRRPKRREIPL KVLVKAVLFACMLMRKTMASRVRVT ILFATETGKSEALAWDLGAL FSCAFNPKVVCMDKYRLSCLEEERL LLVVTSTFGNGDCPGNGEKLKKSLF MLKELNNKFRYAVFGLGSSM YPRFCAFAHDIDQKLSHLGASQLTP MGEGDELSGQEDAFRSWAVQTFKAA CETFDVRGKQHIQIPKLYTS NVTWDPHHYRLVQDSQPLDLSKALS SMHAKNVFTMRLKSRQNLQSPTSSR ATILVELSCEDGQGLNYLPG EHLGVCPGNQPALVQGILERVVDGP TPHQTVRLEALDESGSYWVSDKRLP PCSLSQALTYFLDITTPPTQ LLLQKLAQVATEEPERQRLEALCQP SEYSKWKFTNSPTFLEVLEEFPSLR VSAGFLLSQLPILKPRFYSI SSSRDHMPTEIHLTVAVVTYHTRDG QGPLHHGVCSTWLNSLKPQDPVPCF VRNASGFHLPEDPSHPCILI GPGTGIAPFRSFWQQRLHDSQHKGV RGGRMTLVFGCRRPDEDHIYQEEML EMAQKGVLHAVHTAYSRLPG KPKVYVQDILRQQLASEVLRVLHKE PGHLYVCGDVRMARDVAHTLKQLVA AKLKLNEEQVEDYFFQLKSQ
  KRYHEDIFGAVFPYEAKKDRVAVQP SSLEMSALTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer

Gene Information

• Gene Name: NOS2 nitric oxide synthase 2 [ Homo sapiens (human) ]
• Official Symbol: NOS2
• Synonyms: NOS; INOS; NOS2A; HEP-NOS
• Gene ID: 4843
• mRNA Refseq: NM_000625.4
• Protein Refseq: NP_000616.3
• MIM: 163730
• UniProt ID: P35228