Recombinant Full Length Human NPR1 Protein, C-Flag-tagged

• Cat.No.: NPR1-357HFL
• Product Overview: Recombinant Full Length Human NPR1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP, respectively.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 118.7 kDa
• AA Sequence: MPGPRRPAGSRLRLLLLLLLPPLLL LLRGSHAGNLTVAVVLPLANTSYPW SWARVGPAVELALAQVKARP DLLPGWTVRTVLGSSENALGVCSDT AAPLAAVDLKWEHNPAVFLGPGCVY AAAPVGRFTAHWRVPLLTAG APALGFGVKDEYALTTRAGPSYAKL GDFVAALHRRLGWERQALMLYAYRP GDEEHCFFLVEGLFMRVRDR LNITVDHLEFAEDDLSHYTRLLRTM PRKGRVIYICSSPDAFRTLMLLALE AGLCGEDYVFFHLDIFGQSL QGGQGPAPRRPWERGDGQDVSARQA FQAAKIITYKDPDNPEYLEFLKQLK HLAYEQFNFTMEDVLVNTIP ASFHDGLLLYIQAVTETLAHGGTVT DGENITQRMWNRSFQGVTGYLKIDS SGDRETDFSLWDMDPENGAF RVVLNYNGTSQELVAVSGRKLNWPL GYPPPDIPKCGFDNEDPACNQDHLS TLEVLALVGSLSLLGILIVS FFIYRKMQLEKELASELWRVRWEDV EPSSLERHLRSAGSRLTLSGRGSNY GSLLTTEGQFQVFAKTAYYK GNLVAVKRVNRKRIELTRKVLFELK HMRDVQNEHLTRFVGACTDPPNICI LTEYCPRGSLQDILENESIT LDWMFRYSLTNDIVKGMLFLHNGAI CSHGNLKSSNCVVDGRFVLKITDYG LESFRDLDPEQGHTVYAKKL WTAPELLRMASPPVRGSQAGDVYSF GIILQEIALRSGVFHVEGLDLSPKE IIERVTRGEQPPFRPSLALQ SHLEELGLLMQRCWAEDPQERPPFQ QIRLTLRKFNRENSSNILDNLLSRM EQYANNLEELVEERTQAYLE EKRKAEALLYQILPHSVAEQLKRGE TVQAEAFDSVTIYFSDIVGFTALSA ESTPMQVVTLLNDLYTCFDA VIDNFDVYKVETIGDAYMVVSGLPV RNGRLHACEVARMALALLDAVRSFR IRHRPQEQLRLRIGIHTGPV CAGVVGLKMPRYCLFGDTVNTASRM ESNGEALKIHLSSETKAVLEEFGGF ELELRGDVEMKGKGKVRTYW
  LLGERGSSTRGTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction

Gene Information

• Gene Name: NPR1 natriuretic peptide receptor 1 [ Homo sapiens (human) ]
• Official Symbol: NPR1
• Synonyms: ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• Gene ID: 4881
• mRNA Refseq: NM_000906.4
• Protein Refseq: NP_000897.3
• MIM: 108960
• UniProt ID: P16066