Recombinant Full Length Human NSF Protein, C-Flag-tagged

• Cat.No.: NSF-1227HFL
• Product Overview: Recombinant Full Length Human NSF Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables PDZ domain binding activity and ionotropic glutamate receptor binding activity. Involved in intracellular protein transport; positive regulation of protein catabolic process; and positive regulation of receptor recycling. Located in Golgi apparatus; cytosol; and plasma membrane. Implicated in developmental and epileptic encephalopathy.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 82.4 kDa
• AA Sequence: MAGRSMQAARCPTDELSLTNCAVVN EKDFQSGQHVIVRTSPNHRYTFTLK THPSVVPGSIAFSLPQRKWA GLSIGQEIEVSLYTFDKAKQCIGTM TIEIDFLQKKSIDSNPYDTDKMAAE FIQQFNNQAFSVGQQLVFSF NEKLFGLLVKDIEAMDPSILKGEPA TGKRQKIEVGLVVGNSQVAFEKAEN SSLNLIGKAKTKENRQSIIN PDWNFEKMGIGGLDKEFSDIFRRAF ASRVFPPEIVEQMGCKHVKGILLYG PPGCGKTLLARQIGKMLNAR EPKVVNGPEILNKYVGESEANIRKL FADAEEEQRRLGANSGLHIIIFDEI DAICKQRGSMAGSTGVHDTV VNQLLSKIDGVEQLNNILVIGMTNR PDLIDEALLRPGRLEVKMEIGLPDE KGRLQILHIHTARMRGHQLL SADVDIKELAVETKNFSGAELEGLV RAAQSTAMNRHIKASTKVEVDMEKA ESLQVTRGDFLASLENDIKP AFGTNQEDYASYIMNGIIKWGDPVT RVLDDGELLVQQTKNSDRTPLVSVL LEGPPHSGKTALAAKIAEES NFPFIKICSPDKMIGFSETAKCQAM KKIFDDAYKSQLSCVVVDDIERLLD YVPIGPRFSNLVLQALLVLL KKAPPQGRKLLIIGTTSRKDVLQEM EMLNAFSTTIHVPNIATGEQLLEAL ELLGNFKDKERTTIAQQVKG
  KKVWIGIKKLLMLIEMSLQMDPEYR VRKFLALLREEGASPLDFDTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Protease

Gene Information

• Gene Name: NSF N-ethylmaleimide sensitive factor, vesicle fusing ATPase [ Homo sapiens (human) ]
• Official Symbol: NSF
• Synonyms: SKD2; DEE96; SEC18
• Gene ID: 4905
• mRNA Refseq: NM_006178.4
• Protein Refseq: NP_006169.2
• MIM: 601633
• UniProt ID: P46459