Recombinant Full Length Human NUF2 Protein, His tagged

• Cat.No.: NUF2-02HFL
• Product Overview: Purified recombinant protein of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-464 aa
• Description: This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
• Molecular Mass: 54.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Shipping: Dry Ice

Gene Information

• Gene Name 2: NUF2 NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol 2: NUF2
• Synonyms 2: NUF2; NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae); CDCA1, cell division cycle associated 1; kinetochore protein Nuf2; cancer/testis antigen 106; CT106; NUF2R; hNuf2; hNuf2R; hsNuf2; cell division cycle associated 1; cell division cycle-associated protein 1; CDCA1
• Gene ID 2: 83540
• mRNA Refseq 2: NM_145697
• Protein Refseq 2: NP_663735
• MIM 2: 611772
• UniProt ID 2: Q9BZD4