Recombinant Full Length Human PC Protein, C-Flag-tagged

• Cat.No.: PC-1561HFL
• Product Overview: Recombinant Full Length Human PC Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 127.4 kDa
• AA Sequence: MLKFRTVHGGLRLLGIRRTSTAPAA SPNVRRLEYKPIKKVMVANRGEIAI RVFRACTELGIRTVAIYSEQ DTGQMHRQKADEAYLIGRGLAPVQA YLHIPDIIKVAKENNVDAVHPGYGF LSERADFAQACQDAGVRFIG PSPEVVRKMGDKVEARAIAIAAGVP VVPGTDAPITSLHEAHEFSNTYGFP IIFKAAYGGGGRGMRVVHSY EELEENYTRAYSEALAAFGNGALFV EKFIEKPRHIEVQILGDQYGNILHL YERDCSIQRRHQKVVEIAPA AHLDPQLRTRLTSDSVKLAKQVGYE NAGTVEFLVDRHGKHYFIEVNSRLQ VEHTVTEEITDVDLVHAQIH VAEGRSLPDLGLRQENIRINGCAIQ CRVTTEDPARSFQPDTGRIEVFRSG EGMGIRLDNASAFQGAVISP HYDSLLVKVIAHGKDHPTAATKMSR ALAEFRVRGVKTNIAFLQNVLNNQQ FLAGTVDTQFIDENPELFQL RPAQNRAQKLLHYLGHVMVNGPTTP IPVKASPSPTDPVVPAVPIGPPPAG FRDILLREGPEGFARAVRNH PGLLLMDTTFRDAHQSLLATRVRTH DLKKIAPYVAHNFSKLFSMENWGGA TFDVAMRFLYECPWRRLQEL RELIPNIPFQMLLRGANAVGYTNYP DNVVFKFCEVAKENGMDVFRVFDSL NYLPNMLLGMEAAGSAGGVV EAAISYTGDVADPSRTKYSLQYYMG LAEELVRAGTHILCIKDMAGLLKPT ACTMLVSSLRDRFPDLPLHI HTHDTSGAGVAAMLACAQAGADVVD VAADSMSGMTSQPSMGALVACTRGT PLDTEVPMERVFDYSEYWEG ARGLYAAFDCTATMKSGNSDVYENE IPGGQYTNLHFQAHSMGLGSKFKEV KKAYVEANQMLGDLIKVTPS SKIVGDLAQFMVQNGLSRAEAEAQA EELSFPRSVVEFLQGYIGVPHGGFP EPFRSKVLKDLPRVEGRPGA SLPPLDLQALEKELVDRHGEEVTPE DVLSAAMYPDVFAHFKDFTATFGPL DSLNTRLFLQGPKIAEEFEV ELERGKTLHIKALAVSDLNRAGQRQ VFFELNGQLRSILVKDTQAMKEMHF HPKALKDVKGQIGAPMPGKV
  IDIKVVAGAKVAKGQPLCVLSAMKM ETVVTSPMEGTVRKVHVTKDMTLEG DDLILEIETRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism

Gene Information

• Gene Name: PC pyruvate carboxylase [ Homo sapiens (human) ]
• Official Symbol: PC
• Synonyms: PCB
• Gene ID: 5091
• mRNA Refseq: NM_000920.4
• Protein Refseq: NP_000911.2
• MIM: 608786
• UniProt ID: P11498