Recombinant Full Length Human PIK3C3 Protein, C-Flag-tagged

• Cat.No.: PIK3C3-1722HFL
• Product Overview: Recombinant Full Length Human PIK3C3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 101.4 kDa
• AA Sequence: MGEAEKFHYIYSCDLDINVQLKIGS LEGKREQKSYKAVLEDPMLKFSGLY QETCSDLYVTCQVFAEGKPL ALPVRTSYKAFSTRWNWNEWLKLPV KYPDLPRNAQVALTIWDVYGPGKAV PVGGTTVSLFGKYGMFRQGM HDLKVWPNVEADGSEPTKTPGRTSS TLSEDQMSRLAKLTKAHRQGHMVKV DWLDRLTFREIEMINESEKR SSNFMYLMVEFRCVKCDDKEYGIVY YEKDGDESSPILTSFELVKVPDPQM SMENLVESKHHKLARSLRSG PSDHDLKPNAATRDQLNIIVSYPPT KQLTYEEQDLVWKFRYYLTNQEKAL TKFLKCVNWDLPQEAKQALE LLGKWKPMDVEDSLELLSSHYTNPT VRRYAVARLRQADDEDLLMYLLQLV QALKYENFDDIKNGLEPTKK DSQSSVSENVSNSGINSAEIDSSQI ITSPLPSVSSPPPASKTKEVPDGEN LEQDLCTFLISRACKNSTLA NYLYWYVIVECEDQDTQQRDPKTHE MYLNVMRRFSQALLKGDKSVRVMRS LLAAQQTFVDRLVHLMKAVQ RESGNRKKKNERLQALLGDNEKMNL SDVELIPLPLEPQVKIRGIIPETAT LFKSALMPAQLFFKTEDGGK YPVIFKHGDDLRQDQLILQIISLMD KLLRKENLDLKLTPYKVLATSTKHG FMQFIQSVPVAEVLDTEGSI QNFFRKYAPSENGPNGISAEVMDTY VKSCAGYCVITYILGVGDRHLDNLL LTKTGKLFHIDFGYILGRDP KPLPPPMKLNKEMVEGMGGTQSEQY QEFRKQCYTAFLHLRRYSNLILNLF SLMVDANIPDIALEPDKTVK
  KVQDKFRLDLSDEEAVHYMQSLIDE SVHALFAAVVEQIHKFAQYWRKTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy

Gene Information

• Gene Name: PIK3C3 phosphatidylinositol 3-kinase catalytic subunit type 3 [ Homo sapiens (human) ]
• Official Symbol: PIK3C3
• Synonyms: VPS34; Vps34; hVps34
• Gene ID: 5289
• mRNA Refseq: NM_002647.4
• Protein Refseq: NP_002638.2
• MIM: 602609
• UniProt ID: Q8NEB9