Recombinant Full Length Human POSTN, Isoform 3, His tagged

• Cat.No.: POSTN-820H
• Product Overview: Recombinant Human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-781 aa
• Form: Lyophilized from sterile PBS, 5% Trehalose, pH 7.0 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately >60% will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The recombinant human POSTN consists of 771 amino acids and has a predicted molecular mass of 86.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 66-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: POSTN periostin, osteoblast specific factor [ Homo sapiens ]
• Official Symbol: POSTN
• Synonyms: POSTN; periostin, osteoblast specific factor; periostin; OSF 2; PN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; osteoblast-specific factor 2; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like); OSF2; OSF-2; PDLPOSTN; RP11-412K4.1; MGC119510; MGC119511
• Gene ID: 10631
• mRNA Refseq: NM_001135934
• Protein Refseq: NP_001129406
• MIM: 608777
• UniProt ID: Q15063