Recombinant Full Length Human PPT1 protein, His-tagged

• Cat.No.: PPT1-367H
• Product Overview: Recombinant Full Length Human PPT1 protein(P50897-1)(Met1-Gly306) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-306 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human PPT1 comprises 290 amino acids and has a predicted molecular mass of 32.7 kDa. The apparent molecular mass of the protein is approximately 35-41 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Niemann Pick C1 mistargeting disrupts lysosomal cholesterol homeostasis contributing to neurodegeneration in a Batten disease model (2025)

Gene Information

• Gene Name: PPT1 palmitoyl-protein thioesterase 1 [ Homo sapiens ]
• Official Symbol: PPT1
• Synonyms: PPT1; palmitoyl-protein thioesterase 1; PPT; ceroid lipofuscinosis; neuronal 1; infantile; CLN1; INCL; PPT-1; palmitoyl-protein hydrolase 1; ceroid-palmitoyl-palmitoyl-protein thioesterase 1
• Gene ID: 5538
• mRNA Refseq: NM_000310
• Protein Refseq: NP_000301
• MIM: 600722
• UniProt ID: P50897