Recombinant Full Length Human PYGB Protein, C-Flag-tagged

• Cat.No.: PYGB-1653HFL
• Product Overview: Recombinant Full Length Human PYGB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 96.5 kDa
• AA Sequence: MAKPLTDSEKRKQISVRGLAGLGDV AEVRKSFNRHLHFTLVKDRNVATPR DYFFALAHTVRDHLVGRWIR TQQHYYERDPKRIYYLSLEFYMGRT LQNTMVNLGLQNACDEAIYQLGLDL EELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEFG IFNQKIVNGWQVEEADDWLRYGNPW EKARPEYMLPVHFYGRVEHT PDGVKWLDTQVVLAMPYDTPVPGYK NNTVNTMRLWSAKAPNDFKLQDFNV GDYIEAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATL QDIIRRFKSSKFGCRDPVRTCFETF PDKVAIQLNDTHPALSIPEL MRILVDVEKVDWDKAWEITKKTCAY TNHTVLPEALERWPVSMFEKLLPRH LEIIYAINQRHLDHVAALFP GDVDRLRRMSVIEEGDCKRINMAHL CVIGSHAVNGVARIHSEIVKQSVFK DFYELEPEKFQNKTNGITPR RWLLLCNPGLADTIVEKIGEEFLTD LSQLKKLLPLVSDEVFIRDVAKVKQ ENKLKFSAFLEKEYKVKINP SSMFDVHVKRIHEYKRQLLNCLHVV TLYNRIKRDPAKAFVPRTVMIGGKA APGYHMAKLIIKLVTSIGDV VNHDPVVGDRLKVIFLENYRVSLAE KVIPAADLSQQISTAGTEASGTGNM KFMLNGALTIGTMDGANVEM AEEAGAENLFIFGLRVEDVEALDRK GYNAREYYDHLPELKQAVDQISSGF FSPKEPDCFKDIVNMLMHHD RFKVFADYEAYMQCQAQVDQLYRNP KEWTKKVIRNIACSGKFSSDRTITE YAREIWGVEPSDLQIPPPNI
  PRDSGPTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism

Gene Information

• Gene Name: PYGB glycogen phosphorylase B [ Homo sapiens (human) ]
• Official Symbol: PYGB
• Synonyms: GPBB
• Gene ID: 5834
• mRNA Refseq: NM_002862.4
• Protein Refseq: NP_002853.2
• MIM: 138550
• UniProt ID: P11216