Recombinant Full Length Human PYGM Protein, C-Flag-tagged

• Cat.No.: PYGM-1281HFL
• Product Overview: Recombinant Full Length Human PYGM Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 96.9 kDa
• AA Sequence: MSRPLSDQEKRKQISVRGLAGVENV TELKKNFNRHLHFTLVKDRNVATPR DYYFALAHTVRDHLVGRWIR TQQHYYEKDPKRIYYLSLEFYMGRT LQNTMVNLALENACDEATYQLGLDM EELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEFG IFNQKISGGWQMEEADDWLRYGNPW EKARPEFTLPVHFYGHVEHT SQGAKWVDTQVVLAMPYDTPVPGYR NNVVNTMRLWSAKAPNDFNLKDFNV GGYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATL QDIIRRFKSSKFGCRDPVRTNFDAF PDKVAIQLNDTHPSLAIPEL MRILVDLERMDWDKAWDVTVRTCAY TNHTVLPEALERWPVHLLETLLPRH LQIIYEINQRFLNRVAAAFP GDVDRLRRMSLVEEGAVKRINMAHL CIAGSHAVNGVARIHSEILKKTIFK DFYELEPHKFQNKTNGITPR RWLVLCNPGLAEVIAERIGEDFISD LDQLRKLLSFVDDEAFIRDVAKVKQ ENKLKFAAYLEREYKVHINP NSLFDIQVKRIHEYKRQLLNCLHVI TLYNRIKREPNKFFVPRTVMIGGKA APGYHMAKMIIRLVTAIGDV VNHDPAVGDRLRVIFLENYRVSLAE KVIPAADLSEQISTAGTEASGTGNM KFMLNGALTIGTMDGANVEM AEEAGEENFFIFGMRVEDVDKLDQR GYNAQEYYDRIPELRQVIEQLSSGF FSPKQPDLFKDIVNMLMHHD RFKVFADYEDYIKCQEKVSALYKNP REWTRMVIRNIATSGKFSSDRTIAQ YAREIWGVEPSRQRLPAPDE
  AITRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism

Gene Information

• Gene Name: PYGM glycogen phosphorylase, muscle associated [ Homo sapiens (human) ]
• Official Symbol: PYGM
• Synonyms: GSD5
• Gene ID: 5837
• mRNA Refseq: NM_005609.4
• Protein Refseq: NP_005600.1
• MIM: 608455
• UniProt ID: P11217